UNIT 12.4 Culture of HIV in Monocytes and Macrophages
Published Online: 1 JAN 2006
Copyright © 2005 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Crowe, S., Maslin, C., Ellery, P. and Kedzierska, K. 2006. Culture of HIV in Monocytes and Macrophages. Current Protocols in Immunology. 70:12.4:12.4.1–12.4.13.
- Published Online: 1 JAN 2006
- Published Print: DEC 2005
Monocytes and macrophages constitute important cellular targets for macrophage-tropic (M-tropic) strains of HIV-1 and are believed to play a major role in the pathogenesis of AIDS. This updated unit provides procedures for culturing HIV in these target cells under suspension and adherent cell culture conditions. The unit also provides methods for expanding M-tropic primary isolates of HIV and determining the 50% tissue culture infectious dose (TCID50) of HIV stocks. Curr. Protoc. Immunol. 70:12.4.1-12.4.13. © 2005 by John Wiley & Sons, Inc.