Unit

UNIT 12.4 Culture of HIV in Monocytes and Macrophages

  1. Suzanne Crowe1,
  2. Clare Maslin1,
  3. Philip Ellery1,
  4. Katherine Kedzierska2

Published Online: 1 JAN 2006

DOI: 10.1002/0471142735.im1204s70

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Crowe, S., Maslin, C., Ellery, P. and Kedzierska, K. 2006. Culture of HIV in Monocytes and Macrophages. Current Protocols in Immunology. 70:12.4:12.4.1–12.4.13.

Author Information

  1. 1

    Macfarlane Burnet Institute for Medical Research and Monash University, Melbourne, Australia

  2. 2

    University of Melbourne, Melbourne, Australia

Publication History

  1. Published Online: 1 JAN 2006
  2. Published Print: DEC 2005

Abstract

Monocytes and macrophages constitute important cellular targets for macrophage-tropic (M-tropic) strains of HIV-1 and are believed to play a major role in the pathogenesis of AIDS. This updated unit provides procedures for culturing HIV in these target cells under suspension and adherent cell culture conditions. The unit also provides methods for expanding M-tropic primary isolates of HIV and determining the 50% tissue culture infectious dose (TCID50) of HIV stocks. Curr. Protoc. Immunol. 70:12.4.1-12.4.13. © 2005 by John Wiley & Sons, Inc.