UNIT 12.5 Detection Assays for HIV Proteins

  1. Mario A. Ostrowski,
  2. Tae-Wook Chun,
  3. Sharilyn K. Stanley,
  4. Anthony S. Fauci

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im1205s42

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Ostrowski, M. A., Chun, T.-W., Stanley, S. K. and Fauci, A. S. 2001. Detection Assays for HIV Proteins. Current Protocols in Immunology. 12:12.5.

Author Information

  1. National Institute of Allergy and Infectious Diseases, NIH, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 2001

This is not the most recent version of the article. View current version (1 JAN 2006)


This updated unit describes four assays for detection of HIV proteins including: (1) a quantitative ELISA, (2) a quantitative immunoblotting assay, (3) a qualitative immunofluorescence assay, and (4) a functional assay to measure virus-associated reverse transcriptase activity as an indicator of viral production.