Unit

UNIT 12.6 Detection of HIV DNA and RNA Using PCR

  1. Susan Moir,
  2. Tae-Wook Chun

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im1206s42

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Moir, S. and Chun, T.-W. 2001. Detection of HIV DNA and RNA Using PCR. Current Protocols in Immunology. 42:12.6:12.6.1–12.6.12.

Author Information

  1. National Institute of Allergy and Infectious Diseases, NIH, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 2001

Abstract

This extensively updated unit describes the detection of HIV DNA and RNA using PCR using two basic techniques for quantifying the levels of viral DNA and RNA in infected cells. The schemes for both techniques include isolation of nucleic acids, PCR reactions and detection of amplified products using Southern blotting.