UNIT 12.6 Detection of HIV DNA and RNA Using PCR
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Moir, S. and Chun, T.-W. 2001. Detection of HIV DNA and RNA Using PCR. Current Protocols in Immunology. 42:12.6.1–12.6.12.
- Published Online: 1 MAY 2001
- Published Print: APR 2001
This extensively updated unit describes the detection of HIV DNA and RNA using PCR using two basic techniques for quantifying the levels of viral DNA and RNA in infected cells. The schemes for both techniques include isolation of nucleic acids, PCR reactions and detection of amplified products using Southern blotting.