UNIT 12.9 In Vitro Evaluation of Experimental Agents for Anti-HIV Activity
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Richman, D. D., Johnson, V. A., Mayers, D. M., Shirasaka, T., O'Brien, M. C. and Mitsuya, H. 2001. In Vitro Evaluation of Experimental Agents for Anti-HIV Activity. Current Protocols in Immunology. 8:12.9:12.9.1–12.9.21.
- Published Online: 1 MAY 2001
- Published Print: DEC 1993
This unit presents an assay that has proven useful as an initial screening test is an HIV cytopathic effect (CPE) inhibition assay in which immortalized T cell lines (e.g., ATH8 or MT2) that are profoundly sensitive to the cytopathic effect of certain strains of HIV are utilized as target cells. Additional protocols assess the anti-HIV activity of certain candidate agents by measuring inhibition of syncytium formation or p24 gag protein production by ELISA. Calculation of the 50% inhibitory concentration (IC50) is also presented.