Unit

UNIT 12.10 Vaccinia-Based Reporter Gene Cell-Fusion Assays to Quantitate Functional Interactions of HIV Envelope Glycoprotein with Receptors

  1. Barna Dey,
  2. Edward A. Berger

Published Online: 1 MAY 2003

DOI: 10.1002/0471142735.im1210s54

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Dey, B. and Berger, E. A. 2003. Vaccinia-Based Reporter Gene Cell-Fusion Assays to Quantitate Functional Interactions of HIV Envelope Glycoprotein with Receptors. Current Protocols in Immunology. 54:12.10:12.10.1–12.10.20.

Author Information

  1. National Institutes of Health, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2003
  2. Published Print: APR 2003

Abstract

This unit describes quantitation of functional interactions between HIV envelope glcoprotein and target cell receptors, using assay of cell fusion-dependent reporter gene activation. The method is particularly useful since it isolates the fusion reaction from the rest of the HIV replication cycle, and obviates the need for infectious HIV particles.Reporter Gene Cell Fusion Assays to Quantitate Functional Interactions of HIV Envelope Glycoprotein with Receptors