UNIT 12.12 Immunophenotyping of T Cell Subpopulations in HIV Disease

  1. Pratip K. Chattopadhyay,
  2. Mario Roederer

Published Online: 1 MAR 2005

DOI: 10.1002/0471142735.im1212s65

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Chattopadhyay, P. K. and Roederer, M. 2005. Immunophenotyping of T Cell Subpopulations in HIV Disease. Current Protocols in Immunology. 65:12.12:12.12.1–12.12.15.

Author Information

  1. National Institutes of Health, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAR 2005
  2. Published Print: FEB 2005


This unit illustrates the challenges associated with measurements of T cell subpopulations, and describes how those challenges can be overcome by using polychromatic (5+ color) approaches to flow cytometry. Such approaches are immensely powerful and directly applicable to studies of T cell biology in HIV disease; however, their development requires careful consideration of the antibodies and fluorochromes employed, as discussed here. Furthermore, factors generally known to influence T cell counts can also govern the success or failure of polychromatic experiments; therefore, these factors are reviewed and provide recommendations for minimizing their influence on polychromatic measurements of T cell subsets.


  • Polychromatic flow cytometry;
  • T cell subsets;
  • immunophenotyping;
  • CD4 count