UNIT 12.12 Immunophenotyping of T Cell Subpopulations in HIV Disease
Published Online: 1 MAR 2005
Copyright © 2005 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Chattopadhyay, P. K. and Roederer, M. 2005. Immunophenotyping of T Cell Subpopulations in HIV Disease. Current Protocols in Immunology. 65:12.12:12.12.1–12.12.15.
- Published Online: 1 MAR 2005
- Published Print: FEB 2005
This unit illustrates the challenges associated with measurements of T cell subpopulations, and describes how those challenges can be overcome by using polychromatic (5+ color) approaches to flow cytometry. Such approaches are immensely powerful and directly applicable to studies of T cell biology in HIV disease; however, their development requires careful consideration of the antibodies and fluorochromes employed, as discussed here. Furthermore, factors generally known to influence T cell counts can also govern the success or failure of polychromatic experiments; therefore, these factors are reviewed and provide recommendations for minimizing their influence on polychromatic measurements of T cell subsets.
- Polychromatic flow cytometry;
- T cell subsets;
- CD4 count