Unit

UNIT 12.13 Evaluation of B Cell Function in Patients with HIV

  1. Clarisa M. Buckner,
  2. Lela Kardava,
  3. Susan Moir

Published Online: 1 FEB 2013

DOI: 10.1002/0471142735.im1213s100

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Buckner, C. M., Kardava, L. and Moir, S. 2013. Evaluation of B Cell Function in Patients with HIV. Current Protocols in Immunology. 100:12.13:12.13.1–12.13.18.

Author Information

  1. National Institutes of Health, Bethesda, Maryland

Publication History

  1. Published Online: 1 FEB 2013
  2. Published Print: FEB 2013

Abstract

HIV disease is associated with abnormalities in all major lymphocyte populations, including B cells. B cell dysfunction in HIV infection is largely driven by alterations in the subsets of B cells that circulate in the blood or reside in tissues. Meaningful functional assays are thus dependent on the identification and isolation of B cell subsets present in the starting material. This unit describes several assays designed to phenotype, fractionate, and assess functional properties of B cells that circulate in the blood of HIV-infected individuals. The four protocols, which have been adapted from standard techniques, are tailored to evaluate B cells in peripheral blood mononuclear cells (PBMCs) of individuals infected with HIV, but can also be applied to other disease settings and PBMCs isolated from healthy individuals. Curr. Protoc. Immunol. 100:12.13.1-12.13.18. © 2013 by John Wiley & Sons, Inc.

Keywords:

  • B cell;
  • subset isolation;
  • immunophenotyping;
  • HIV;
  • ELISPOT;
  • proliferation;
  • CFSE