Unit

UNIT 13.2 Alternative Pathway Evaluation

  1. Patricia C. Giclas

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im1302s09

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Giclas, P. C. 2001. Alternative Pathway Evaluation. Current Protocols in Immunology. 9:13.2:13.2.1–13.2.9.

Author Information

  1. National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAR 1994

Abstract

The alternative pathway of complement shares its terminal components (C3 and C5 through 9) with the classical pathway, but has several unique components, including factors D, B, and P (properdin). This unit presents methods for assaying total alternative pathway activity and the activity of factors B and D. Radial immunodiffusion (RID) can also be used to measure factor D, B, and P concentrations.