UNIT 13.2 Alternative Pathway Evaluation
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Giclas, P. C. 2001. Alternative Pathway Evaluation. Current Protocols in Immunology. 9:13.2:13.2.1–13.2.9.
- Published Online: 1 MAY 2001
- Published Print: MAR 1994
The alternative pathway of complement shares its terminal components (C3 and C5 through 9) with the classical pathway, but has several unique components, including factors D, B, and P (properdin). This unit presents methods for assaying total alternative pathway activity and the activity of factors B and D. Radial immunodiffusion (RID) can also be used to measure factor D, B, and P concentrations.