UNIT 13.2 Alternative Pathway Evaluation

  1. Patricia C. Giclas

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im1302s09

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Giclas, P. C. 2001. Alternative Pathway Evaluation. Current Protocols in Immunology. 9:13.2:13.2.1–13.2.9.

Author Information

  1. National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAR 1994


The alternative pathway of complement shares its terminal components (C3 and C5 through 9) with the classical pathway, but has several unique components, including factors D, B, and P (properdin). This unit presents methods for assaying total alternative pathway activity and the activity of factors B and D. Radial immunodiffusion (RID) can also be used to measure factor D, B, and P concentrations.