UNIT 13.3 Isolation and Purification of C3 from Human Plasma

  1. Lynda D. O'Rear,
  2. Gordon D. Ross

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im1303s14

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

O'Rear, L. D. and Ross, G. D. 2001. Isolation and Purification of C3 from Human Plasma. Current Protocols in Immunology. 10:13.3:13.3.1–13.3.16.

Author Information

  1. University of Louisville, Louisville, Kentucky

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 1995


The alternative pathway of complement shares its terminal components (C3 and C5 through 9) with the classical pathway, but has several unique components, including factors D, B, and P (properdin). This unit presents methods for assaying total alternative pathway activity and the activity of factors B and D. Radial immunodiffusion (RID) can also be used to measure factor D, B, and P concentrations.