UNIT 13.3 Isolation and Purification of C3 from Human Plasma
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
O'Rear, L. D. and Ross, G. D. 2001. Isolation and Purification of C3 from Human Plasma. Current Protocols in Immunology. 10:13.3:13.3.1–13.3.16.
- Published Online: 1 MAY 2001
- Published Print: JUN 1995
The alternative pathway of complement shares its terminal components (C3 and C5 through 9) with the classical pathway, but has several unique components, including factors D, B, and P (properdin). This unit presents methods for assaying total alternative pathway activity and the activity of factors B and D. Radial immunodiffusion (RID) can also be used to measure factor D, B, and P concentrations.