Unit

UNIT 13.6 Assays for the Mannan-Binding Lectin Pathway

  1. Mihaela Gadjeva,
  2. Steffen Thiel,
  3. Jens C. Jensenius

Published Online: 1 FEB 2004

DOI: 10.1002/0471142735.im1306s58

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Gadjeva, M., Thiel, S. and Jensenius, J. C. 2004. Assays for the Mannan-Binding Lectin Pathway. Current Protocols in Immunology. 58:13.6:13.6.1–13.6.8.

Author Information

  1. University of Aarhus, Aarhus, Denmark

Publication History

  1. Published Online: 1 FEB 2004
  2. Published Print: DEC 2003

Abstract

This unit contains protocols that can be used to measure mannan-binding lectin (MBL) levels and MBL pathway activity in human plasma or serum. Using a modification of the conventional ELISA, the detection reagent used in the methods described (e.g., an antibody or streptavidin) is labeled with Eu3+ instead of an enzyme. The sensitizing reagent used in these assays employ the use of anti-MBL antibody or mannan. The unit also includes an MBL/MASP complex activity assay to evaluate MBL activity in serum or plasma samples, which allows for the measurement of the specific activity of the MBL pathway.

Keywords:

  • complement;
  • lectin pathway activity;
  • mannan binding lectin;
  • mannose binding lectin (MBL)