UNIT 13.6 Assays for the Mannan-Binding Lectin Pathway
Published Online: 1 FEB 2004
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Gadjeva, M., Thiel, S. and Jensenius, J. C. 2004. Assays for the Mannan-Binding Lectin Pathway. Current Protocols in Immunology. 58:13.6:13.6.1–13.6.8.
- Published Online: 1 FEB 2004
- Published Print: DEC 2003
This unit contains protocols that can be used to measure mannan-binding lectin (MBL) levels and MBL pathway activity in human plasma or serum. Using a modification of the conventional ELISA, the detection reagent used in the methods described (e.g., an antibody or streptavidin) is labeled with Eu3+ instead of an enzyme. The sensitizing reagent used in these assays employ the use of anti-MBL antibody or mannan. The unit also includes an MBL/MASP complex activity assay to evaluate MBL activity in serum or plasma samples, which allows for the measurement of the specific activity of the MBL pathway.
- lectin pathway activity;
- mannan binding lectin;
- mannose binding lectin (MBL)