UNIT 14.1 Isolation of Murine Macrophages

  1. Anne H. Fortier (peritoneum)1,
  2. Lydia A. Falk (bone marrow)2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im1401s11

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Fortier, A. H. and Falk, L. A. 2001. Isolation of Murine Macrophages. Current Protocols in Immunology. 11:14.1:14.1.1–14.1.9.

Author Information

  1. 1

    EntreMed, Inc., Rockville, Maryland

  2. 2

    Food and Drug Administration, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 1994

This is not the most recent version of the article. View current version (2 NOV 2015)


Macrophages isolated from murine peritoneal cavity, bone marrow, and spleen are suitable samples for studying the activation properties of this immunologically important cell type. This unit describes the isolation of murine macrophages from the peritoneal cavity under inflammatory and noninflammatory conditions. To investigate macrophage activation using a more homogeneous population of cells, macrophages derived from immature progenitor cells in bone marrow can be studied. A second protocol describes the isolation of bone marrow-derived progenitor cells and propagation of these immature macrophages by CSFs or IL-3.