UNIT 14.1 Isolation of Murine Macrophages
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Fortier, A. H. and Falk, L. A. 2001. Isolation of Murine Macrophages. Current Protocols in Immunology. 11:14.1:14.1.1–14.1.9.
- Published Online: 1 MAY 2001
- Published Print: SEP 1994
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Macrophages isolated from murine peritoneal cavity, bone marrow, and spleen are suitable samples for studying the activation properties of this immunologically important cell type. This unit describes the isolation of murine macrophages from the peritoneal cavity under inflammatory and noninflammatory conditions. To investigate macrophage activation using a more homogeneous population of cells, macrophages derived from immature progenitor cells in bone marrow can be studied. A second protocol describes the isolation of bone marrow-derived progenitor cells and propagation of these immature macrophages by CSFs or IL-3.