Unit

UNIT 14.1 The Isolation and Characterization of Murine Macrophages

  1. Xia Zhang,
  2. Ricardo Goncalves,
  3. David M. Mosser

Published Online: 1 NOV 2008

DOI: 10.1002/0471142735.im1401s83

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Zhang, X., Goncalves, R. and Mosser, D. M. 2008. The Isolation and Characterization of Murine Macrophages. Current Protocols in Immunology. 83:14.1:14.1.1–14.1.14.

Author Information

  1. Department of Cell Biology and Molecular Genetics, The Maryland Pathogen Research Institute, University of Maryland, College Park, Maryland

Publication History

  1. Published Online: 1 NOV 2008
  2. Published Print: NOV 2008

Abstract

Macrophages are mononuclear phagocytes that are widely distributed throughout the body. These cells can contribute to development and homeostasis and participate in innate and adaptive immune responses. The physiology of macrophages can vary tremendously depending on the environment in which they reside and the local stimuli to which they are exposed. Macrophages are prodigious secretory cells, and in that role can promote and regulate immune responses and contribute to autoimmune pathologies. Macrophages are highly phagocytic, and in this capacity have long been considered to be essential immune effector cells. The important roles of macrophages in maintaining homeostasis and in contributing to tissue remodeling and wound healing is sometimes overlooked because of their vital role in host defense. Curr. Protoc. Immunol. 83:14.1.1-14.1.14. © 2008 by John Wiley & Sons, Inc.

Keywords:

  • colony stimulating factor;
  • peritoneal cavity;
  • lung;
  • bone marrow;
  • flow cytometry;
  • biomarkers