Unit

UNIT 14.2 Characterization of Murine Macrophages

  1. Mary C. Riedy1,
  2. Carleton C. Stewart2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im1402s14

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Riedy, M. C. and Stewart, C. C. 2001. Characterization of Murine Macrophages. Current Protocols in Immunology. 14:14.2:14.2.1–14.2.8.

Author Information

  1. 1

    National Institute of Arthritis and Musculoskeletal and Skin Diseases, Bethesda, Maryland

  2. 2

    Roswell Park Cancer Institute, Buffalo, New York

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 1995

This is not the most recent version of the article. View current version (1 NOV 2008)

Abstract

This unit describes the phenotypic characterization of macrophages through the use of fluorescence-labeled monoclonal antibodies (MAbs) that have specificity for certain extracellular membrane proteins expressed by macrophages. Although macrophages have no distinctive cell-surface antigen that allows for their enrichment, a specific set of markers can be used to distinguish macrophages from other cell types in a heterogeneous population of cells. The method can also be easily applied to cells from other sources, e.g., peritoneal exudates or spleen. Through the use of image or flow cytometry, these cells can be identified and (with flow cytometry) sorted out from a heterogeneous population for further culture or analysis.