UNIT 14.4 Activation of Murine Macrophages

  1. Anne H. Fortier

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im1404s11

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Fortier, A. H. 2001. Activation of Murine Macrophages. Current Protocols in Immunology. 11:14.4:14.4.1–14.4.5.

Author Information

  1. EntreMed, Inc., Rockville, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 1994

This is not the most recent version of the article. View current version (1 NOV 2008)


Macrophages can act as potent effector cells of the immune response. Cytokine signals generated during an immune response activate macrophages in a complex cascade of events. Activated macrophages are qualitatively different from mature resident tissue macrophages and from immature inflammatory macrophages. Activated macrophages acquire new abilities to eliminate intracellular pathogens (bacterial or parasitic) and extracellular tumor or parasite targets. This unit describes the production of macrophages that display cytotoxicity against intracellular parasites, starting from resident peritoneal macrophages. An Alternate Protocol describes the generation of macrophages with tumoricidal activity, starting from inflammatory peritoneal macrophages.