UNIT 14.5 Oxidative Metabolism of Murine Macrophages
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Green, S. J., Aniagolu, J. and Raney, J. J. 2001. Oxidative Metabolism of Murine Macrophages. Current Protocols in Immunology. 12:14.5:14.5.1–14.5.11.
- Published Online: 1 MAY 2001
- Published Print: DEC 1994
This unit describes two simple and straightforward microassays that can be used to measure the levels of NO2− and O2−, respectively that are generated by a small number of immunologically-stimulated macrophages. Detection of these products may be used to identify cytokine(s), microbe(s), or microbial products(s) that regulate oxidative metabolism and effector activity. Although a number of other reliable and sensitive methods are available for assaying these two oxidative metabolites, the microassays described here require little time, technical expertise, or materials. It is not clear at present whether human monocytes/macrophages can also produce NO2−. These protocols are therefore restricted to mouse macrophages.