Unit

UNIT 14.5 Oxidative Metabolism of Murine Macrophages

  1. Shawn J. Green,
  2. Jacinta Aniagolu,
  3. Jennifer J. Raney

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im1405s12

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Green, S. J., Aniagolu, J. and Raney, J. J. 2001. Oxidative Metabolism of Murine Macrophages. Current Protocols in Immunology. 12:14.5:14.5.1–14.5.11.

Author Information

  1. EntreMed, Inc., Rockville, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 1994

Abstract

This unit describes two simple and straightforward microassays that can be used to measure the levels of NO2 and O2, respectively that are generated by a small number of immunologically-stimulated macrophages. Detection of these products may be used to identify cytokine(s), microbe(s), or microbial products(s) that regulate oxidative metabolism and effector activity. Although a number of other reliable and sensitive methods are available for assaying these two oxidative metabolites, the microassays described here require little time, technical expertise, or materials. It is not clear at present whether human monocytes/macrophages can also produce NO2. These protocols are therefore restricted to mouse macrophages.