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UNIT 14.6 Measurement of Bacterial Ingestion and Killing by Macrophages

  1. Priscilla A. Campbell,
  2. Beth P. Canono,
  3. Douglas A. Drevets

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im1406s12

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Campbell, P. A., Canono, B. P. and Drevets, D. A. 2001. Measurement of Bacterial Ingestion and Killing by Macrophages. Current Protocols in Immunology. 12:14.6:14.6.1–14.6.13.

Author Information

  1. National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 1994

This is not the most recent version of the article. View current version (1 APR 2015)

Literature Cited

  1. Literature Cited
  2. Key Reference
  • Drevets, D.A. and Campbell, P.A. 1991a. Roles of complement and complement receptor type 3 in phagocytosis of Listeria monocytogenes by inflammatory mouse peritoneal macrophages. Infect. Immun. 59:2645-2652.
  • Drevets, D.A. and Campbell, P.A. 1991b. Macrophage phagocytosis: Use of fluorescence microscopy to distinguish between extracellular and intracellular bacteria. J. Immunol. Methods 142:31-38.
  • Drevets, D.A., Canono, B.P., and Campbell, P.A. 1992. Listericidal and nonlistericidal mouse macrophages differ in complement receptor type 3-mediated phagocytosis of L. monocytogenes and in preventing escape of the bacteria into the cytoplasm. J. Leukocyte Biol. 52:70-79.
  • Drevets, D.A., Canono, B.P., Leenen, P.J.M., and Campbell, P.A. 1994. Gentamicin kills intracellular Listeria monocytogenes. Infect. Immun. 62:2222-2228.
  • Fattorossi, A., Nisini, R., Pizzolo, J.G., and D'Amelio, R. 1989. New, simple flow cytometry technique to distinguish between internalized and membrane-bound particles in phagocytosis. Cytometry 10:320-325.
  • Flesch, I.E.A. and Kaufmann, S.H.E. 1991. Mechanisms involved in mycobacterial growth inhibition by gamma interferon-activated bone marrow macrophages: Role of reactive nitrogen intermediates. Infect. Immun. 59:3213-3218.
  • Green, S.J., Meltzer, M.S., Hibbs, Jr. J.B., and Nacy, C.A. 1990. Activated macrophages destroy intracellular Leishmania major amastigotes by an l-arginine-dependent killing mechanism. J. Immunol. 144:278-283.
  • Klebanoff, S.J. 1992. Oxygen metabolites from phagocytes. In Inflammation: Basic Principles and Clinical Correlates. (J.I. Gallin, I.M. Goldstein, and R. Snyderman, eds.) pp. 541-588. Raven Press, New York.
  • Leenen, P.J.M. and Campbell, P.A. 1993. Heterogeneity of mononuclear phagocytes—An interpretative review. Blood Cell Biochem. 5:29-85.
  • Payne, N.R. and Horwitz, M.A. 1987. Phagocytosis of Legionella pneumophila is mediated by human monocyte complement receptors. J. Exp. Med. 166:1377-1389.
  • Peck, R. 1985. A one-plate assay for macrophage bactericidal activity. J. Immunol. Methods 82:131-140.
  • Schlesinger, L.S., Bellinger-Kawahara, C.G., Payne, N.R., and Horwitz, M.A. 1990. Phagocytosis of Mycobacterium tuberculosis is mediated by human monocyte complement receptors and complement component C3. J. Immunol. 144:2771-2780.

Key Reference

  1. Literature Cited
  2. Key Reference
  • Drevets, D.A. and Campbell, P.A. 1991b. See above.

Describes method for determining critically whether bacteria are merely bound to the surface of bacteria, or have been internalized—a question clearly important for bactericidal activity as well, as bacteria bound to macrophage surfaces are not killed by the macrophage unless internalized.