Unit

UNIT 14.7 Assay for Macrophage-Mediated Anti-Tumor Cytotoxicity

  1. George W. Cox

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im1407s12

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Cox, G. W. 2001. Assay for Macrophage-Mediated Anti-Tumor Cytotoxicity. Current Protocols in Immunology. 12:14.7:14.7.1–14.7.10.

Author Information

  1. National Cancer Institute, Frederick, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 1994

Abstract

This unit describes a procedure for determining ability of mouse macrophages to lyse tumor cells in vitro. The Basic Protocol outlines a three-stage assay that includes: (1) culture of macrophages (freshly explanted from mice or grown from a cell line) with suspected activating reagents; (2) extensive washing of the cultured macrophages to remove residual reagents, followed by incubation with [111In]-labeled tumor cells to allow lysis to occur; and (3) collection of cell-free culture supernatants and measurement of cytolytic activity as a function of 111In released from tumor cells destroyed by activated macrophages. The Support Protocol outlines a method for radiolabeling tumor cells with [111In]oxine.