UNIT 14.8 Measurement of Fcγ Receptor–Mediated Binding and Phagocytosis
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Politis, A. D. and Vogel, S. N. 2001. Measurement of Fcγ Receptor–Mediated Binding and Phagocytosis. Current Protocols in Immunology. 14:14.8.
- Published Online: 1 MAY 2001
- Published Print: MAR 1995
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Macrophages are perhaps best recognized for their ability to phagocytose foreign particles. Phagocytosis can be nonspecific, but is often dramatically augmented with the aid of receptors that recognize the Fc portion of antibodies bound to the intruder. Macrophages utilize receptors that recognize IgG (FcγR), which is a major opsonic mediator of phagocytosis. This unit describes two functional assays that quantify macrophage FcγR surface expression. In each assay, IgG-opsonized sheep red blood cells (SRBC) labeled with Na251CrO4 are used to detect interaction with macrophages. The Basic Protocol is used to measure phagocytosis of target cells. The Alternate Protocol is used to measure FcγR-mediated binding of target cells to macrophages. A choice of opsonin in both methods allows for some discrimination between subclasses of FcγRs that are responsible for the observed results.