UNIT 14.8 Measurement of Fcγ Receptor–Mediated Binding and Phagocytosis
Published Online: 1 SEP 2005
Copyright © 2005 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Politis, A. D. and Vogel, S. N. 2005. Measurement of Fcγ Receptor–Mediated Binding and Phagocytosis. Current Protocols in Immunology. 68:14.8:14.8.1–14.8.9.
- Published Online: 1 SEP 2005
- Published Print: AUG 2005
This unit describes functional assays that quantify macrophage FcγR surface expression. In each assay, IgG-opsonized sheep red blood cells (SRBC) labeled with Na251CrO4 are used to detect interaction with macrophages. The Basic Protocol measures phagocytosis of target cells following treatment of macrophages with agents known to modulate FcγR expression. The Alternate Protocol describes a method that can be used to measure FcγR-mediated binding of target cells to macrophages. A choice of opsonin in both methods allows for some discrimination between subclasses of FcγRs that are responsible for the observed results.