UNIT 14.11 Isolation and Functional Use of Human NK T Cells
Published Online: 1 FEB 2003
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Exley, M. A., Balk, S. P. and Wilson, S. B. 2003. Isolation and Functional Use of Human NK T Cells. Current Protocols in Immunology. 52:14.11:14.11.1–14.11.13.
- Published Online: 1 FEB 2003
- Published Print: DEC 2002
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Isolation and Functional Use of Human NK T Cells (Mark A. Exley andSteven P. Balk, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts; S. Brian Wilson, Massachusetts General Hospital, Harvard Medical School, Cambridge, Massachusetts). This unit describes methods for the isolation and functional analysis of human invariant NK T cells. The first protocol uses standard multicolor flow cytometry methods and staining reagents specific for V to generate invariant NK T cell clones. Support protocols for secondary stimulations and for rapid expansion of invariant NK T cell clones and functional analysis of these cells are also included.