Unit

UNIT 14.11 Isolation and Functional Use of Human NK T Cells

  1. Mark A. Exley1,
  2. Steven P. Balk1,
  3. S. Brian Wilson2

Published Online: 1 FEB 2003

DOI: 10.1002/0471142735.im1411s52

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Exley, M. A., Balk, S. P. and Wilson, S. B. 2003. Isolation and Functional Use of Human NK T Cells. Current Protocols in Immunology. 52:14.11:14.11.1–14.11.13.

Author Information

  1. 1

    Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts

  2. 2

    Massachusetts General Hospital Harvard Medical School, Cambridge, Massachusetts

Publication History

  1. Published Online: 1 FEB 2003
  2. Published Print: DEC 2002

This is not the most recent version of the article. View current version (1 AUG 2010)

Abstract

Isolation and Functional Use of Human NK T Cells (Mark A. Exley andSteven P. Balk, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts; S. Brian Wilson, Massachusetts General Hospital, Harvard Medical School, Cambridge, Massachusetts). This unit describes methods for the isolation and functional analysis of human invariant NK T cells. The first protocol uses standard multicolor flow cytometry methods and staining reagents specific for V to generate invariant NK T cell clones. Support protocols for secondary stimulations and for rapid expansion of invariant NK T cell clones and functional analysis of these cells are also included.