Unit

UNIT 14.14 Measuring Autophagy in Macrophages

  1. James Harris1,
  2. Orla Hanrahan1,
  3. Sergio A. De Haro2

Published Online: 1 NOV 2009

DOI: 10.1002/0471142735.im1414s87

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Harris, J., Hanrahan, O. and De Haro, S. A. 2009. Measuring Autophagy in Macrophages. Current Protocols in Immunology. 87:14.14.1–14.14.13.

Author Information

  1. 1

    School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland

  2. 2

    University of New Mexico School of Medicine, Albuquerque, New Mexico

Publication History

  1. Published Online: 1 NOV 2009
  2. Published Print: NOV 2009

Abstract

Macroautophagy is a conserved intracellular homeostatic mechanism for the degradation of cytosolic constituents. Autophagy can promote cell survival by providing essential amino acids from the breakdown of macromolecules during periods of nutrient deprivation, and can remove damaged or excess organelles, such as mitochondria and peroxisomes. More recently, autophagy has been shown to play an important role in innate and adaptive immune responses to pathogenic bacteria in macrophages and dendritic cells. This unit presents protocols for the measurement of autophagy in macrophages. Curr. Protoc. Immunol. 87:14.14.1-14.14.13. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • autophagosome;
  • LC3;
  • monodansylcadaverine;
  • Atg;
  • p62;
  • rapamycin