UNIT 14.14 Measuring Autophagy in Macrophages
Published Online: 1 NOV 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Harris, J., Hanrahan, O. and De Haro, S. A. 2009. Measuring Autophagy in Macrophages. Current Protocols in Immunology. 87:14.14:14.14.1–14.14.13.
- Published Online: 1 NOV 2009
- Published Print: NOV 2009
Macroautophagy is a conserved intracellular homeostatic mechanism for the degradation of cytosolic constituents. Autophagy can promote cell survival by providing essential amino acids from the breakdown of macromolecules during periods of nutrient deprivation, and can remove damaged or excess organelles, such as mitochondria and peroxisomes. More recently, autophagy has been shown to play an important role in innate and adaptive immune responses to pathogenic bacteria in macrophages and dendritic cells. This unit presents protocols for the measurement of autophagy in macrophages. Curr. Protoc. Immunol. 87:14.14.1-14.14.13. © 2009 by John Wiley & Sons, Inc.