Unit

UNIT 14.15 Monocyte and Neutrophil Isolation and Migration Assays

  1. Simon Yona1,
  2. Richard Hayhoe2,
  3. Inbal Avraham-Davidi3

Published Online: 1 FEB 2010

DOI: 10.1002/0471142735.im1415s88

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Yona, S., Hayhoe, R. and Avraham-Davidi, I. 2010. Monocyte and Neutrophil Isolation and Migration Assays. Current Protocols in Immunology. 88:14.15:14.15.1–14.15.14.

Author Information

  1. 1

    The Weizmann Institute of Science, Rehovot, Israel

  2. 2

    University of Cambridge, Cambridge, United Kingdom

  3. 3

    The Hebrew University–Hadassah Medical School, Jerusalem, Israel

Publication History

  1. Published Online: 1 FEB 2010
  2. Published Print: FEB 2010

Abstract

This unit describes methods for isolating mouse monocytes and neutrophils, as well as in vitro protocols for measuring cell migration and polarization. The method employed here for the isolation of naïve phagocytes overcomes many of the difficulties previously encountered concerning phagocyte activation. Three in vitro protocols are provided for the analysis of cell migration, one requiring no specialized equipment, one requiring the modified Boyden chamber, and the other employing a flow chamber, which measures cell adhesion, rolling, and migration. Finally, a method is provided for imaging polarized cells by confocal microscopy. Curr. Protoc. Immunol. 88:14.15.1-14.15.14. © 2010 by John Wiley & Sons, Inc.

Keywords:

  • monocyte;
  • neutrophil;
  • chemotaxis;
  • flow chamber;
  • cell polarization