UNIT 14.17 Measurement of Myeloid Cell Immune Suppressive Activity
Published Online: 1 NOV 2010
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Dolcetti, L., Peranzoni, E. and Bronte, V. 2010. Measurement of Myeloid Cell Immune Suppressive Activity. Current Protocols in Immunology. 91:14.17:14.17.1–14.17.25.
- Published Online: 1 NOV 2010
- Published Print: NOV 2010
This unit presents simple methods to assess the immunosuppressive properties of immunoregulatory cells of myeloid origin, such as myeloid-derived suppressor cells (MDSCs), both in vitro and in vivo. These methods are general and could be adapted to test the impact of different suppressive populations on T cell activation, proliferation, and cytotoxic activity; moreover they could be useful to assess the influence exerted on immune suppressive pathways by genetic modifications, chemical inhibitors, and drugs. Curr. Protoc. Immunol. 91:14.17.1-14.17.25. © 2010 by John Wiley & Sons, Inc.
- immunomagnetic sorting;
- thymidine incorporation;
- chromium release test;
- adoptive cell transfer