UNIT 14.17 Measurement of Myeloid Cell Immune Suppressive Activity

  1. Luigi Dolcetti,
  2. Elisa Peranzoni,
  3. Vincenzo Bronte

Published Online: 1 NOV 2010

DOI: 10.1002/0471142735.im1417s91

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Dolcetti, L., Peranzoni, E. and Bronte, V. 2010. Measurement of Myeloid Cell Immune Suppressive Activity. Current Protocols in Immunology. 91:14.17:14.17.1–14.17.25.

Author Information

  1. Istituto Oncologico Veneto, IRCCS, Padua, Italy

Publication History

  1. Published Online: 1 NOV 2010
  2. Published Print: NOV 2010


This unit presents simple methods to assess the immunosuppressive properties of immunoregulatory cells of myeloid origin, such as myeloid-derived suppressor cells (MDSCs), both in vitro and in vivo. These methods are general and could be adapted to test the impact of different suppressive populations on T cell activation, proliferation, and cytotoxic activity; moreover they could be useful to assess the influence exerted on immune suppressive pathways by genetic modifications, chemical inhibitors, and drugs. Curr. Protoc. Immunol. 91:14.17.1-14.17.25. © 2010 by John Wiley & Sons, Inc.


  • MDSC;
  • immunosuppression;
  • immunomagnetic sorting;
  • thymidine incorporation;
  • chromium release test;
  • adoptive cell transfer