Unit

UNIT 14.18 Measurement of Tumor Cytolysis by Macrophages

  1. Mónica Escórcio-Correia,
  2. Thorsten Hagemann

Published Online: 1 FEB 2011

DOI: 10.1002/0471142735.im1418s92

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Escórcio-Correia, M. and Hagemann, T. 2011. Measurement of Tumor Cytolysis by Macrophages. Current Protocols in Immunology. 92:14.18:14.18.1–14.18.11.

Author Information

  1. Centre for Cancer and Inflammation, Institute of Cancer, Queen Mary University of London, Barts and the London School of Medicine and Dentistry, London, United Kingdom

Publication History

  1. Published Online: 1 FEB 2011
  2. Published Print: FEB 2011

Abstract

This unit describes two different protocols for the measurement of tumor cytolysis by macrophages. Traditionally, cytotoxicity assays have relied on the use of radioactive isotopes. In Basic Protocol 1, cytotoxic activity is measured by the release into the culture supernatant of a radioisotope that had been incorporated by the target cell and is released upon cell death. This poses a problem for some cell lines in which spontaneous isotope release occurs in the absence of effector cell cytotoxicity. In Basic Protocol 2, a nonradioactive approach is used to measure cytolysis that relies on the fluorescence staining of tumor cells with cell-death markers. It also provides the obvious advantage of avoiding the use of hazardous radioactive materials. Curr. Protoc. Immunol. 92:14.18.1-14.18.11. © 2011 by John Wiley & Sons, Inc.

Keywords:

  • macrophages;
  • cytolysis;
  • tumor;
  • annexin V;
  • 7-AAD;
  • CFDA-SE;
  • [111In]oxine