Unit

UNIT 14.19 In Vivo and Ex Vivo Protocols for Measuring the Killing of Extracellular Pathogens by Macrophages

  1. Eva Medina,
  2. Oliver Goldmann

Published Online: 1 FEB 2011

DOI: 10.1002/0471142735.im1419s92

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Medina, E. and Goldmann, O. 2011. In Vivo and Ex Vivo Protocols for Measuring the Killing of Extracellular Pathogens by Macrophages. Current Protocols in Immunology. 92:14.19:14.19.1–14.19.17.

Author Information

  1. Infection Immunology Research Group, Helmholtz Centre for Infection Research, Braunschweig, Germany

Publication History

  1. Published Online: 1 FEB 2011
  2. Published Print: FEB 2011

Abstract

This unit describes a series of in vivo/ex vivo combined protocols for investigating the interactions (adhesion, phagocytosis, and killing) of extracellular bacteria with peritoneal murine macrophages. It includes steps needed for in vivo infection of murine peritoneal macrophages after intraperitoneal inoculation with the pathogen of interest, as well as the measurement of bacteria associated with or truly internalized by these phagocytic cells. Several protocols for the ex vivo measurement of the ability of peritoneal macrophages to kill the microorganisms that have been ingested during the in vivo infection assay are included. Curr. Protoc. Immunol. 92:14.19.1-14.19.17. © 2011 by John Wiley & Sons, Inc.

Keywords:

  • macrophages;
  • extracellular pathogens;
  • Streptococcus pyogenes;
  • phagocytosis assay;
  • killing assay;
  • fluorescence microscopy;
  • flow cytometry