Unit

UNIT 14.20 Measurement of Macrophage Growth and Differentiation

  1. Violeta Chitu,
  2. Yee-Guide Yeung,
  3. Wenfeng Yu,
  4. Sayan Nandi,
  5. E. Richard Stanley

Published Online: 1 FEB 2011

DOI: 10.1002/0471142735.im1420s92

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Chitu, V., Yeung, Y.-G., Yu, W., Nandi, S. and Stanley, E. R. 2011. Measurement of Macrophage Growth and Differentiation. Current Protocols in Immunology. 92:14.20:14.20.1–14.20.26.

Author Information

  1. Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, New York

Publication History

  1. Published Online: 1 FEB 2011
  2. Published Print: FEB 2011

Abstract

This unit provides protocols for measuring the abundance and growth of macrophage precursors in agar cultures and the proliferation of isolated mature macrophages in vitro, by either direct cell counting or by DNA measurement. Methods for the immunohistochemical identification of macrophages and the determination of their proliferative status in vivo by immunofluorescence are also included. It also describes methods for characterization of macrophage differentiation through the immunofluorescence analysis of cell-surface expression of CSF-1 receptor. Curr. Protoc. Immunol. 92:14.20.1-14.20.26. © 2011 by John Wiley & Sons, Inc.

Keywords:

  • macrophages;
  • microglia;
  • myeloid;
  • differentiation;
  • CFU-M assay;
  • macrophage surface markers;
  • CSF-1 receptor