UNIT 14.20 Measurement of Macrophage Growth and Differentiation
Published Online: 1 FEB 2011
Copyright © 2011 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Chitu, V., Yeung, Y.-G., Yu, W., Nandi, S. and Stanley, E. R. 2011. Measurement of Macrophage Growth and Differentiation. Current Protocols in Immunology. 92:14.20:14.20.1–14.20.26.
- Published Online: 1 FEB 2011
- Published Print: FEB 2011
This unit provides protocols for measuring the abundance and growth of macrophage precursors in agar cultures and the proliferation of isolated mature macrophages in vitro, by either direct cell counting or by DNA measurement. Methods for the immunohistochemical identification of macrophages and the determination of their proliferative status in vivo by immunofluorescence are also included. It also describes methods for characterization of macrophage differentiation through the immunofluorescence analysis of cell-surface expression of CSF-1 receptor. Curr. Protoc. Immunol. 92:14.20.1-14.20.26. © 2011 by John Wiley & Sons, Inc.
- CFU-M assay;
- macrophage surface markers;
- CSF-1 receptor