Unit

UNIT 14.21 Measurement of Type I Interferon Production

  1. Rosalind E. Seeds1,2,
  2. Joanna L. Miller2

Published Online: 1 FEB 2011

DOI: 10.1002/0471142735.im1421s92

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Seeds, R. E. and Miller, J. L. 2011. Measurement of Type I Interferon Production. Current Protocols in Immunology. 92:14.21:14.21.1–14.21.11.

Author Information

  1. 1

    Sir William Dunn School of Pathology, University of Oxford, United Kingdom

  2. 2

    Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, United Kingdom

Publication History

  1. Published Online: 1 FEB 2011
  2. Published Print: FEB 2011

Abstract

The Basic Protocol in this unit describes measurement of murine interferon (IFN)α/β by intracellular staining for these cytokines and detection by flow cytometry. Alternate protocols detail an enzyme-linked immunoabsorbent assay (ELISA) for IFNα and a biological assay to measure IFN. The FACS assay allows measurement of IFNα/β production by defined cell populations, while ELISA measures secreted IFNα. The bioassay measures functional antiviral activity and is the most sensitive of the assays discussed. These assays therefore provide complementary methods to assess IFN production by murine cells. Curr. Protoc. Immunol. 92:14.21.1-14.21.11. © 2011 by John Wiley & Sons, Inc.

Keywords:

  • intracellular cytokine staining;
  • ELISA;
  • bioassay;
  • type I interferon;
  • cytokines