UNIT 14.21 Measurement of Type I Interferon Production
Published Online: 1 FEB 2011
Copyright © 2011 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Seeds, R. E. and Miller, J. L. 2011. Measurement of Type I Interferon Production. Current Protocols in Immunology. 92:14.21:14.21.1–14.21.11.
- Published Online: 1 FEB 2011
- Published Print: FEB 2011
The Basic Protocol in this unit describes measurement of murine interferon (IFN)α/β by intracellular staining for these cytokines and detection by flow cytometry. Alternate protocols detail an enzyme-linked immunoabsorbent assay (ELISA) for IFNα and a biological assay to measure IFN. The FACS assay allows measurement of IFNα/β production by defined cell populations, while ELISA measures secreted IFNα. The bioassay measures functional antiviral activity and is the most sensitive of the assays discussed. These assays therefore provide complementary methods to assess IFN production by murine cells. Curr. Protoc. Immunol. 92:14.21.1-14.21.11. © 2011 by John Wiley & Sons, Inc.
- intracellular cytokine staining;
- type I interferon;