UNIT 14.23 Measuring the Killing of Intracellular Pathogens: Leishmania
Published Online: 1 APR 2011
Copyright © 2011 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Stenger, S. and van Zandbergen, G. 2011. Measuring the Killing of Intracellular Pathogens: Leishmania. Current Protocols in Immunology. 93:14.23:14.23.1–14.23.12.
- Published Online: 1 APR 2011
- Published Print: APR 2011
Macrophages are professional phagocytes serving as a first line of defence against pathogenic organisms. Macrophages are equipped with efficient effector functions to kill invading microorganisms. The first important mechanism of macrophage host-defence is phagocytosis of pathogens. Subsequently, internalized pathogens are targeted for destruction in maturating phagolysosomal compartments. This process is mediated by lysosomal proteases and an acidified compartment. To investigate macrophages' killing potential in this chapter, we describe an assay based on human primary cells infected with the obligatory intracellular parasite Leishmania. For this pathogen the macrophage has a dual role. The parasite can use macrophages for its intracellular multiplication, but at the same time host macrophages, upon stimulation, can kill the parasite. Curr. Protoc. Immunol. 93:14.23.1-14.23.12. © 2011 by John Wiley & Sons, Inc.
- killing assay;