UNIT 14.25 Measurement of Macrophage-Mediated Killing of Intracellular Bacteria, Including Francisella and Mycobacteria
Published Online: 1 APR 2011
Copyright © 2011 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Elkins, K. L., Cowley, S. C. and Conlan, J. W. 2011. Measurement of Macrophage-Mediated Killing of Intracellular Bacteria, Including Francisella and Mycobacteria. Current Protocols in Immunology. 93:14.25.1–14.25.13.
- Published Online: 1 APR 2011
- Published Print: APR 2011
Macrophages activated by T cell cytokines are a critical defense mechanism against intracellular bacterial pathogens. This unit presents two general methods for assessing the capacity of mouse macrophages, activated with either soluble cytokines or whole immune T lymphocytes, to control or reduce numbers of intracellular bacteria residing within them. “Measurement of killing” is inferred from a reduction in the number of colony-forming units (cfu) of bacteria at the end of a culture period, compared to the input numbers of cfu at initiation of culture, to the peak numbers of cfu measured during culture, or to a control group in which killing is expected to be poor. Curr. Protoc. Immunol. 93:14.25.1-14.25.13. © 2011 by John Wiley & Sons, Inc.
- intracellular bacteria;
- intracellular killing;
- T lymphocytes