Miscellaneous
UNIT 14.28 Analysis of Gene Expression and Gene Silencing in Human Macrophages
Published Online: 1 FEB 2012
DOI: 10.1002/0471142735.im1428s96
Copyright © 2012 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
Additional Information
How to Cite
Martinez, F. O. 2012. Analysis of Gene Expression and Gene Silencing in Human Macrophages. Current Protocols in Immunology. 96:14.28.1–14.28.23.
Publication History
- Published Online: 1 FEB 2012
- Published Print: FEB 2012
- Abstract
- Article
- Figures
- References
This unit describes how to execute a gene expression study with human macrophages. It includes protocols for human macrophage preparation, RNA extraction, real-time PCR analysis, and microarray analysis. The unit also includes a protocol for gene silencing in human macrophages. Altering gene expression can be useful to study the contribution of the gene to macrophage function or even expression of other genes. Curr. Protoc. Immunol. 96:14.28.1-14.28.23. © 2012 by John Wiley & Sons, Inc.
Keywords: buffy coat; monocyte; macrophage; microarray; transcriptome; real-time PCR; SYBR Green RT PCR; siRNA