Unit

UNIT 14.28 Analysis of Gene Expression and Gene Silencing in Human Macrophages

  1. Fernando O. Martinez

Published Online: 1 FEB 2012

DOI: 10.1002/0471142735.im1428s96

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Martinez, F. O. 2012. Analysis of Gene Expression and Gene Silencing in Human Macrophages. Current Protocols in Immunology. 96:14.28:14.28.1–14.28.23.

Author Information

  1. Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom

Publication History

  1. Published Online: 1 FEB 2012
  2. Published Print: FEB 2012

Abstract

This unit describes how to execute a gene expression study with human macrophages. It includes protocols for human macrophage preparation, RNA extraction, real-time PCR analysis, and microarray analysis. The unit also includes a protocol for gene silencing in human macrophages. Altering gene expression can be useful to study the contribution of the gene to macrophage function or even expression of other genes. Curr. Protoc. Immunol. 96:14.28.1-14.28.23. © 2012 by John Wiley & Sons, Inc.

Keywords:

  • buffy coat;
  • monocyte;
  • macrophage;
  • microarray;
  • transcriptome;
  • real-time PCR;
  • SYBR Green RT PCR;
  • siRNA