UNIT 14.32 Comparing Individual NK Cell Activity In Vitro
Published Online: 1 FEB 2013
Copyright © 2013 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Sierich, H. and Eiermann, T. 2013. Comparing Individual NK Cell Activity In Vitro. Current Protocols in Immunology. 100:14.32:14.32.1–14.32.11.
- Published Online: 1 FEB 2013
- Published Print: FEB 2013
Natural killer (NK) cells play an important role in the innate immune system by eliminating infected and mutated cells. Their cytotoxic capacities vary markedly among individuals. The cytotoxic activity can be measured in peripheral blood mononuclear cells (PBMCs) using the NK cell–specific target cell line K562. In this chapter, we present a protocol for the standardization and normalization of cell preparation and NK cell cytotoxicity measurement in a 51Cr-release assay. By following these protocols, it is possible to compare the NK cell activity of numerous—if necessary selected—individuals in vitro. Curr. Protoc. Immunol. 100:14.32.1-14.32.11. © 2013 by John Wiley & Sons, Inc.
- 51Cr release assay;