Unit

UNIT 14.33 Measuring Intravascular Migration of Mouse Ly6Clow Monocytes In Vivo Using Intravital Microscopy

  1. L.M. Carlin1,2,5,
  2. C. Auffray3,4,5,
  3. F. Geissmann1,2,3

Published Online: 1 APR 2013

DOI: 10.1002/0471142735.im1433s101

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Carlin, L., Auffray, C. and Geissmann, F. 2013. Measuring Intravascular Migration of Mouse Ly6Clow Monocytes In Vivo Using Intravital Microscopy. Current Protocols in Immunology. 101:14.33:14.33.1–14.33.16.

Author Information

  1. 1

    Centre for Molecular & Cellular Biology of Inflammation, King's College London, London, United Kingdom

  2. 2

    Peter Gorer Department of Immunobiology, King's College London, London, United Kingdom

  3. 3

    INSERM U838, Institut Necker, Paris Descartes University, Paris, France

  4. 4

    CNRS UMR8104, INSERM U1016, Institut Cochin, Paris Descartes University, Paris, France

  5. 5

    These authors contributed equally to this work

Publication History

  1. Published Online: 1 APR 2013
  2. Published Print: APR 2013

Abstract

This unit describes methods for intravital imaging of monocytes in the vasculature of the dermis and the mesentery in vivo using fluorescent reporter mice, fluorescent dyes, and antibodies. Cx3cr1gfp/gfp (or +), Rag2−/−, Il2rg−/− mice expressing eGFP at the locus of the Cx3cr1 gene, on the Rag2−/− Il2rg−/− C57Bl/6 background, are used. Although aimed at specifically tracking Ly6Clow monocytes, these protocols could readily be adapted to investigate the interaction of other blood leukocytes with the vascular endothelium by use of other fluorescent reporter mice and fluorescently labeled antibodies. Curr. Protoc. Immunol. 101:14.33.1-14.33.16. © 2013 by John Wiley & Sons, Inc.

Keywords:

  • Ly6Clow monocytes;
  • intravital microscopy;
  • cell tracking;
  • confocal microscopy;
  • blood vessels;
  • in vivo