UNIT 14.35 Isolation and Analysis of Mouse Microglial Cells
Published Online: 4 FEB 2014
Copyright © 2013 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Garcia, J. A., Cardona, S. M. and Cardona, A. E. 2014. Isolation and Analysis of Mouse Microglial Cells. Current Protocols in Immunology. 104:14.35:14.35.1–14.35.15.
- Published Online: 4 FEB 2014
Microglia are mononuclear phagocytes that make up about 10% of the central nervous system (CNS). They are known for their surveillant behavior, which involves continuous monitoring of neural tissue by extending and retracting their processes. Microglial cells are derived from myeloid progenitor cells and play important roles in homeostasis as well as inflammatory and immune responses in the brain. This unit describes several microglial cell isolation protocols that can be easily adapted for projects requiring a rapid and efficient analysis of mouse microglial cells by flow cytometry. Methods for visualizing microglial cells using in situ immunohistochemistry and immunochemistry in free-floating sections are also included. Curr. Protoc. Immunol. 104:14.35.1-14.35.15. © 2014 by John Wiley & Sons, Inc.
- mononuclear cells;
- microglial isolation;
- myeloid cells;
- Percoll gradients;
- flow cytometry;