UNIT 14.35 Isolation and Analysis of Mouse Microglial Cells

  1. Jenny A. Garcia,
  2. Sandra M. Cardona,
  3. Astrid E. Cardona

Published Online: 4 FEB 2014

DOI: 10.1002/0471142735.im1435s104

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Garcia, J. A., Cardona, S. M. and Cardona, A. E. 2014. Isolation and Analysis of Mouse Microglial Cells. Current Protocols in Immunology. 104:14.35:14.35.1–14.35.15.

Author Information

  1. The University of Texas at San Antonio, San Antonio, Texas

Publication History

  1. Published Online: 4 FEB 2014


Microglia are mononuclear phagocytes that make up about 10% of the central nervous system (CNS). They are known for their surveillant behavior, which involves continuous monitoring of neural tissue by extending and retracting their processes. Microglial cells are derived from myeloid progenitor cells and play important roles in homeostasis as well as inflammatory and immune responses in the brain. This unit describes several microglial cell isolation protocols that can be easily adapted for projects requiring a rapid and efficient analysis of mouse microglial cells by flow cytometry. Methods for visualizing microglial cells using in situ immunohistochemistry and immunochemistry in free-floating sections are also included. Curr. Protoc. Immunol. 104:14.35.1-14.35.15. © 2014 by John Wiley & Sons, Inc.


  • mononuclear cells;
  • microglial isolation;
  • immunostaining;
  • CX3CR1/GFP;
  • CNS;
  • myeloid cells;
  • microglia;
  • Percoll gradients;
  • flow cytometry;
  • histochemistry