UNIT 15.1 Experimental Autoimmune Encephalomyelitis in the Mouse

  1. Stephen D. Miller,
  2. William J. Karpus

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im1501s19

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Miller, S. D. and Karpus, W. J. 2001. Experimental Autoimmune Encephalomyelitis in the Mouse. Current Protocols in Immunology. 15:15.1.

Author Information

  1. Northwestern University Medical School, Chicago, Illinois

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 1996

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This unit details the materials and methods required for both active induction and adoptive transfer of experimental autoimmune encephalomyelitis (EAE) in the SJL mouse strain using intact proteins or peptides from the two major myelin proteins: proteolipid protein (PLP) and myelin basic protein (MBP). Detailed materials and methods required for the purification of both PLP and MBP are also described. Modifications of the specified protocols may be necessary for efficient induction of active or adoptive EAE in other mouse strains.