UNIT 15.1 Experimental Autoimmune Encephalomyelitis in the Mouse

  1. Stephen D. Miller,
  2. William J. Karpus

Published Online: 1 MAY 2007

DOI: 10.1002/0471142735.im1501s77

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Miller, S. D. and Karpus, W. J. 2007. Experimental Autoimmune Encephalomyelitis in the Mouse. Current Protocols in Immunology. 78:15.1:15.1.1–15.1.18.

Author Information

  1. Northwestern University, Chicago, Illinois

Publication History

  1. Published Online: 1 MAY 2007
  2. Published Print: MAY 2007

This is not the most recent version of the article. View current version (1 FEB 2010)


This unit details the materials and methods required for both active induction and adoptive transfer of experimental autoimmune encephalomyelitis (EAE) in the SJL mouse strain using intact proteins or peptides from the two major myelin proteins: proteolipid protein (PLP) and myelin basic protein (MBP). Detailed materials and methods required for the purification of both PLP and MBP are also described. Modifications of the specified protocols may be necessary for efficient induction of active or adoptive EAE in other mouse strains.


  • CFA;
  • complete Freund's adjuvant; CNS;
  • central nervous system; EAE;
  • experimental autoimmune encephalomyelitis; MBP;
  • myelin basic protein; MOG;
  • myelin oligodendrocyte glycoprotein; PLP;
  • proteolipid protein