UNIT 15.1 Experimental Autoimmune Encephalomyelitis in the Mouse

  1. Stephen D. Miller1,
  2. William J. Karpus1,
  3. Todd Scott Davidson2

Published Online: 1 FEB 2010

DOI: 10.1002/0471142735.im1501s88

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Miller, S. D., Karpus, W. J. and Davidson, T. S. 2010. Experimental Autoimmune Encephalomyelitis in the Mouse. Current Protocols in Immunology. 88:15.1:15.1.1–15.1.20.

Author Information

  1. 1

    Northwestern University, Evanston, Illinois

  2. 2

    NIAID, NIH, Bethesda, Maryland

Publication History

  1. Published Online: 1 FEB 2010
  2. Published Print: FEB 2010


This unit details the materials and methods required for both active induction and adoptive transfer of experimental autoimmune encephalomyelitis (EAE) in the SJL mouse strain using intact proteins or peptides from the two major myelin proteins: proteolipid protein (PLP) and myelin basic protein (MBP). Detailed materials and methods required for the purification of both PLP and MBP are also described. A protocol for isolating CNS-infiltrating lymphocytes in EAE mice is included. Modifications of the specified protocols may be necessary for efficient induction of active or adoptive EAE in other mouse strains. Curr. Protoc. Immunol. 88:15.1.1-15.1.20. © 2010 by John Wiley & Sons, Inc.


  • CFA;
  • complete Freund's adjuvant;
  • CNS;
  • central nervous system;
  • EAE;
  • experimental autoimmune encephalomyelitis;
  • MBP;
  • myelin basic protein;
  • MOG;
  • myelin oligodendrocyte glycoprotein;
  • PLP;
  • proteolipid protein