UNIT 15.2 Experimental Autoimmune Encephalomyelitis in the Rat
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Swanborg, R. H. and Stepaniak, J. A. 2001. Experimental Autoimmune Encephalomyelitis in the Rat. Current Protocols in Immunology. 19:15.2:15.2.1–15.2.14.
- Published Online: 1 MAY 2001
- Published Print: SEP 1996
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This unit details the materials and methods required for both active induction and adoptive transfer of experimental autoimmune encephalomyelitis (EAE) in the SJL mouse strain using intact proteins or peptides from the two major myelin proteins: proteolipid protein (PLP) and myelin basic protein (MBP). Detailed materials and methods required for the purification of both PLP and MBP are also described. Modifications of the specified protocols may be necessary for efficient induction of active or adoptive EAE in other mouse strains.