Unit

UNIT 15.2 Experimental Autoimmune Encephalomyelitis in the Rat

  1. Mark Mannie1,
  2. Robert H. Swanborg2,
  3. Jolie A. Stepaniak3

Published Online: 1 APR 2009

DOI: 10.1002/0471142735.im1502s85

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Mannie, M., Swanborg, R. H. and Stepaniak, J. A. 2009. Experimental Autoimmune Encephalomyelitis in the Rat. Current Protocols in Immunology. 85:15.2:15.2.1–15.2.15.

Author Information

  1. 1

    Department of Microbiology and Immunology, East Carolina University, Brody School of Medicine, Greenville, North Carolina

  2. 2

    Wayne State University, Detroit, Michigan

  3. 3

    Henry Ford Community College, Dearborn, Michigan

Publication History

  1. Published Online: 1 APR 2009
  2. Published Print: APR 2009

Abstract

There are several diverse rat models of experimental autoimmune encephalomyelitis (EAE) that can be used to investigate the pathogenesis and regulation of autoimmunity against CNS myelin. The disease course of these models ranges from an acute monophasic disease with limited demyelination to a chronic relapsing or chronic progressive course marked by severe demyelination. These models enable the study of encephalitogenic T cells and demyelinating antibody specific for major neuroantigens such as myelin basic protein (MBP), myelin oligodendrocyte glycoprotein (MOG), or proteolipid protein (PLP), among other important CNS autoantigens. Overall, this unit provides an overview of common methods for induction of active and passive EAE, assessment and analysis of clinical disease, preparation and purification of myelin basic protein, and derivation of neuroantigen-specific rat T cell lines. This unit also provides a brief discussion of the basic characteristics of these models. Curr. Protoc. Immunol. 85:15.2.1-15.2.15. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • animal models;
  • autoimmune disease;
  • central nervous system;
  • demyelination;
  • experimental autoimmune encephalomyelitis;
  • EAE;
  • multiple sclerosis;
  • rat