Unit

UNIT 15.7 Experimental Autoimmune Thyroiditis in the Mouse

  1. Yi-chi M. Kong

Published Online: 1 AUG 2007

DOI: 10.1002/0471142735.im1507s78

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Kong, Y.-c. M. 2007. Experimental Autoimmune Thyroiditis in the Mouse. Current Protocols in Immunology. 78:15.7.1–15.7.21.

Author Information

  1. Wayne State University School of Medicine, Detroit, Michigan

Publication History

  1. Published Online: 1 AUG 2007
  2. Published Print: AUG 2007

Abstract

Experimental autoimmune thyroiditis (EAT) in mice is an excellent model for Hashimoto's thyroiditis (HT). It is induced with thyroglobulin (Tg), a known thyroid autoantigen that is common to both mouse and human and for which several conserved, thyroiditogenic epitopes have been identified. This unit describes induction and evaluation of EAT using thyroid histology and in vitro proliferative response assays. An ELISA is presented to detect the level of antibody to mouse thyroglobulin (MTg). To induce EAT, either bacterial lipopolysaccharide (LPS) or supplemented complete Freund's adjuvant (CFA) can be used as adjuvant. A support protocol for preparing MTg is included. The T cell proliferation assay can be used to examine the antigenicity of synthetic peptides derived from MTg or heterologous Tg. EAT can be adoptively transferred utilizing cells that have been expanded in vitro, as described. A protocol is provided for inducing tolerance using deaggregated MTg; induction of tolerance requires larger amounts of MTg but efficiently suppresses EAT development. Also included is a protocol to demonstrate the role of regulatory T cells in mediating tolerance. A protocol to delineate HLA association with HT is illustrated using HLA class II transgenic mice. Curr. Protoc. Immunol. 78:15.7.1-15.7.21. © 2007 by John Wiley & Sons, Inc.

Keywords:

  • autoimmune thyroiditis;
  • experimental autoimmune thyroiditis;
  • EAT;
  • HLA class II transgenes;
  • HLA-DR3 association;
  • regulatory T cells in EAT