Unit

UNIT 15.14 Autoimmune Myocarditis, Valvulitis, and Cardiomyopathy

  1. Jennifer M. Myers1,
  2. DeLisa Fairweather2,
  3. Sally A. Huber3,
  4. Madeleine W. Cunningham1

Published Online: 1 APR 2013

DOI: 10.1002/0471142735.im1514s101

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Myers, J. M., Fairweather, D., Huber, S. A. and Cunningham, M. W. 2013. Autoimmune Myocarditis, Valvulitis, and Cardiomyopathy. Current Protocols in Immunology. 101:15.14:15.14.1–15.14.51.

Author Information

  1. 1

    University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma

  2. 2

    Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland

  3. 3

    University of Vermont, Colchester, Vermont

Publication History

  1. Published Online: 1 APR 2013
  2. Published Print: APR 2013

Abstract

Myocarditis and valvulitis are inflammatory diseases affecting myocardium and valve. Myocarditis, a viral-induced disease of myocardium, may lead to dilated cardiomyopathy and loss of heart function. Valvulitis leads to deformed heart valves and altered blood flow in rheumatic heart disease. Animal models recapitulating these diseases are important in understanding the human condition. Cardiac myosin is a major autoantigen in heart, and antibodies and T cells to cardiac myosin are evident in inflammatory heart diseases. This unit is a practical guide to induction and evaluation of experimental autoimmune myocarditis (EAM) in several mouse strains and the Lewis rat. Purification protocols for cardiac myosin and protocols for induction of EAM by cardiac myosin and its myocarditis-producing peptides, and coxsackievirus CVB3, are defined. Protocols for assessment of myocarditis and valvulitis in humans and animal models provide methods to define functional autoantibodies targeting cardiac myosin, β-adrenergic, and muscarinic receptors, and their deposition in tissues. Curr. Protoc. Immunol. 101:15.14.1-15.14.51. © 2013 by John Wiley & Sons, Inc.

Keywords:

  • myocarditis;
  • rheumatic fever;
  • autoimmunity;
  • coxsackievirus;
  • cardiac myosin