UNIT 15.19 Induction of TNBS Colitis in Mice

  1. Frank Scheiffele,
  2. Ivan J. Fuss

Published Online: 1 AUG 2002

DOI: 10.1002/0471142735.im1519s49

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Scheiffele, F. and Fuss, I. J. 2002. Induction of TNBS Colitis in Mice. Current Protocols in Immunology. 49:15.19:15.19.1–15.19.14.

Author Information

  1. National Institute of Allergy and Infectious Disease, NIH, Bethesda, Maryland

Publication History

  1. Published Online: 1 AUG 2002
  2. Published Print: JUL 2002


The animal model described in this unit is one of several that have been used to study the immunopathogenesis of inflammatory bowel disease. The model employs the use of 2,4,6-trinitrobenzenesulfonic acid (TNBS), which induces severe colonic inflammation when administered intrarectally in SJL/J mice. The colitis which results from this procedure presents clinical and histopathological findings that resemble those seen in Crohn's disease. The unit describes the critical parameters needed for successful induction of TNBS-colitis as well methods for monitoring and grading disease levels. A support protocol for isolating lamina propria mononuclear cells from mouse colons is also included.