UNIT 15.24 Mouse Models of Atherosclerosis

Genetically altered mice carrying mutations of genes encoding crucial components of the immune system and lipid metabolism have been widely used to study the role of immune responses and inflammation in atherosclerosis. These mice are often fed a diet, with a high content of cholesterol and saturated fat in order to induce hypercholesterolemia and arterial lesions. We review the different mouse models of atherosclerosis, type of diets, and techniques to measure lipid deposition and lesion size in the arterial walls. Moreover, the methods used to determine the presence of the immune cells in atherosclerotic lesions are also described here. Curr. Protoc. Immunol. 96:15.24.1-15.24.23. © 2012 by John Wiley & Sons, Inc.

Keywords: atherosclerosis; mice; diet; lipids; staining