Unit

UNIT 15.26 Glomerulonephritis Induced by Heterologous Anti-GBM Globulin as a Planted Foreign Antigen

  1. Dragana Odobasic1,4,
  2. Joanna R. Ghali1,4,
  3. Kim M. O'Sullivan1,
  4. Stephen R. Holdsworth1,2,
  5. A. Richard Kitching1,2,3

Published Online: 1 AUG 2014

DOI: 10.1002/0471142735.im1526s106

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Odobasic, D., Ghali, J. R., O'Sullivan, K. M., Holdsworth, S. R. and Kitching, A. R. 2014. Glomerulonephritis Induced by Heterologous Anti-GBM Globulin as a Planted Foreign Antigen. Current Protocols in Immunology. 106:15.26:15.26.1–15.26.20.

Author Information

  1. 1

    Centre for Inflammatory Diseases, Monash University Department of Medicine, Clayton, Australia

  2. 2

    Department of Nephrology, Monash Health, Clayton, Australia

  3. 3

    Department of Paediatric Nephrology, Monash Health, Clayton, Australia

  4. 4

    These authors contributed equally to this work

Publication History

  1. Published Online: 1 AUG 2014

Abstract

The glomerulonephritides are diseases characterized by immune-mediated glomerular inflammation. Most severe and rapidly progressive forms of glomerulonephritis feature the participation of injurious leukocytes that localize to glomeruli. This unit describes classical models of rapidly progressive glomerulonephritis in mice, induced by injecting heterologous globulin (raised in sheep) that binds to the glomerular basement membrane. These models have been particularly useful in defining the participation of effector leukocytes in severe glomerular disease. In these models, injury typically occurs in two phases. In the initial, heterologous phase, injury is mediated by the globulin bound within the glomerulus acting as an antibody. The later, autologous phase of injury is mediated by the host's adaptive immunity to the heterologous globulin now functioning as a planted foreign antigen within glomeruli. As autologous phase injury is driven by immunity to sheep globulin, assessment of antigen-specific systemic immunity to sheep globulin is critical when using this model. Curr. Protoc. Immunol. 106:15.26.1-15.26.20. © 2014 by John Wiley & Sons, Inc.

Keywords:

  • glomerulonephritis;
  • cell-mediated immunity;
  • planted antigen;
  • T lymphocytes