UNIT 16.2 Presenting Exogenous Antigen to T Cells
Published Online: 1 FEB 2010
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Harding, C. V. and Ramachandra, L. 2010. Presenting Exogenous Antigen to T Cells. Current Protocols in Immunology. 88:16.2:16.2.1–16.2.18.
- Published Online: 1 FEB 2010
- Published Print: FEB 2010
Antigen processing and presentation experiments can be done with a wide variety of antigen-presenting cells (APCs). Most experiments will use one of the “professional” APC types: dendritic cells (DCs), macrophages, and B lymphocytes. Other types of cells may be used for antigen presentation in some circumstances. Each type of professional APC has an important antigen-presentation function, but the different APC types contribute to different aspects of the immune response. Therefore, selection of an APC type for study must include consideration of the stage or aspect of immune response that is to be modeled in the experiment. An important technical distinction for some types of experiments is whether the APCs are adherent or nonadherent, since this dictates the procedures that must be used to wash the cells as the medium is changed. Curr. Protoc. Immunol. 88:16.2.1-16.2.18. © 2010 by John Wiley & Sons, Inc.
- antigen presenting cells;
- antigen processing;
- antigen presentation;
- T lymphocyte;
- T hybridoma cells