UNIT 17.2 Monitoring Antigen-Specific T Cells Using MHC-Ig Dimers

  1. Jonathan P. Schneck,
  2. Jill E. Slansky,
  3. Sean M. O'Herrin,
  4. Tim F. Greten

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im1702s35

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Schneck, J. P., Slansky, J. E., O'Herrin, S. M. and Greten, T. F. 2001. Monitoring Antigen-Specific T Cells Using MHC-Ig Dimers. Current Protocols in Immunology. 35:17.2:17.2.1–17.2.17.

Author Information

  1. Johns Hopkins University, Baltimore, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 2000


The lack of high affinity reagents has made distinguishing T cells on the basis of antigen specificity difficult to accomplish. This unit provides protocols that utilize innovations in molecular design to permit construction of soluble multivalent MHC complexes (MHC-Ig dimers) with high avidity for cognate T cell receptors. MHC-Ig dimers display stable binding properties when they interact with antigen-specific T cells thus allowing their use in the staining of antigen-specific T cells by flow cytometry. Methods for constructing and detecting these MHC-Ig dimers are included along with protocols for applying their use for the quantitation of antigen-specific T cells.