UNIT 17.2 Monitoring Antigen-Specific T Cells Using MHC-Ig Dimers
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Schneck, J. P., Slansky, J. E., O'Herrin, S. M. and Greten, T. F. 2001. Monitoring Antigen-Specific T Cells Using MHC-Ig Dimers. Current Protocols in Immunology. 35:17.2:17.2.1–17.2.17.
- Published Online: 1 MAY 2001
- Published Print: FEB 2000
The lack of high affinity reagents has made distinguishing T cells on the basis of antigen specificity difficult to accomplish. This unit provides protocols that utilize innovations in molecular design to permit construction of soluble multivalent MHC complexes (MHC-Ig dimers) with high avidity for cognate T cell receptors. MHC-Ig dimers display stable binding properties when they interact with antigen-specific T cells thus allowing their use in the staining of antigen-specific T cells by flow cytometry. Methods for constructing and detecting these MHC-Ig dimers are included along with protocols for applying their use for the quantitation of antigen-specific T cells.