UNIT 17.4 In Situ Staining Using MHC Class I Tetramers
Published Online: 1 JAN 2005
Copyright © 2004 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Skinner, P. J. and Haase, A. T. 2005. In Situ Staining Using MHC Class I Tetramers. Current Protocols in Immunology. 64:17.4:17.4.1–17.4.9.
- Published Online: 1 JAN 2005
- Published Print: DEC 2004
The invention of MHC-tetramer technology to label antigen-specific T cells has lead to a greatly enhanced understanding of T lymphocyte biology. This protocol describes the use of MHC class I tetramers to stain antigen-specific T cells in tissue sections. In situ tetramer staining (IST) can be used to determine the localization, abundance, and phenotype of antigen-specific T cells in native environments and in three-dimensional space. IST is broadly applicable because it can be used to stain essentially any antigen-specific T cell in any tissue for which MHC tetramers are available. IST can be combined with histochemistry and/or immunohistochemistry to permit visualization and characterization of antigen-specific T cells relative to other cell types in stained tissue sections. Thus, IST is a useful and valuable component of MHC-tetramer technology.
- In situ tetramer staining (IST);
- MHC class I tetramers;
- CD8+ T cells;
- confocal microscopy