Unit

UNIT 17.5 Preparation of Stable Single-Chain Trimers Engineered with Peptide, β2 Microglobulin, and MHC Heavy Chain

  1. Ted Hansen,
  2. Y.Y. Lawrence Yu,
  3. Daved H. Fremont

Published Online: 1 NOV 2009

DOI: 10.1002/0471142735.im1705s87

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Hansen, T., Yu, Y. L. and Fremont, D. H. 2009. Preparation of Stable Single-Chain Trimers Engineered with Peptide, β2 Microglobulin, and MHC Heavy Chain. Current Protocols in Immunology. 87:17.5:17.5.1–17.5.17.

Author Information

  1. Washington University School of Medicine, St. Louis, Missouri

Publication History

  1. Published Online: 1 NOV 2009
  2. Published Print: NOV 2009

Abstract

This unit describes a method for constructing a class I MHC molecule with a bound peptide as a single polypeptide chain, termed SCT, for single chain trimer. The component organization of the SCT appears to be widely applicable to different mouse or human MHC class I isotypes bound by different antigenic peptides. The enhanced peptide occupancy afforded by the SCT format makes these molecules effective reagents as DNA vaccines, multimeric staining reagents to enumerate CD8 T cells, and probes of lymphocyte biology. Curr. Protoc. Immunol. 87:17.5.1-17.5.17. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • vaccines;
  • tetramers;
  • lymphocyte development;
  • recombinant MHC/peptide complexes